Open AccessHsa_circ_0031787 promotes cell proliferation and invasion in colorectal cancer
Author(s) -
Luo Min,
Peng Tianshu,
Lin Renjing,
Gu Liyao,
He Yongheng
Publication year - 2021
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23807
Subject(s) - colorectal cancer , cell growth , wnt signaling pathway , cancer research , in vivo , circular rna , biology , cancer , chemistry , medicine , downregulation and upregulation , gene , signal transduction , microbiology and biotechnology , genetics
Objective Circular RNA (circRNA) has been found to be involved in regulating tumor development. However, the roles and underlying mechanisms of circRNA in colorectal cancer (CRC) development remain unclear. In this study, we investigated the effects of hsa_circ_0031787 on CRC.a Methods Aberrant circRNA expression was explored by the Gene Expression Omnibus (GEO) database, and hsa_circ_0031787 was selected for further study. Hsa_circ_0031787 expression was determined in CRC tissues and cell lines by qRT‐PCR. Cell proliferation was measured by Edu and colony formation assays. Cell invasion was tested by Transwell assays. Results Hsa_circ_0031787 expression levels in CRC were significantly increased and correlated with advanced TNM stage and lymph node metastasis in CRC patients. Functional assays showed that hsa_circ_0031787 suppression reduced CRC cell proliferation and invasion in vitro and reduced tumor growth in vivo. Furthermore, hsa_circ_0031787 suppression reduced activation of the Wnt/β‐catenin axis in CRC. Conclusions Our results showed that hsa_circ_0031787 may function as an oncogenic circRNA in CRC progression, thus providing a new potential therapeutic target.