Open AccessShort‐term biological variation and Reference change values of urinary 8‐oxo‐7,8‐dihydro‐2'‐deoxyguanosine and 8‐oxo‐7,8‐dihydroguanosine
Author(s) -
Zhang Ruiping,
Yu Songlin,
Wang Danchen,
Yin Yicong,
Yu Jialei,
Cao Yongtong,
Qiu Ling
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23522
Subject(s) - urine , creatinine , urinary system , morning , chemistry , 8 hydroxy 2' deoxyguanosine , urology , medicine , oxidative stress , dna oxidation , oxidative damage
Abstract Aim The DNA and RNA oxidative damage products urinary 8‐oxo‐7,8‐dihydro‐2'‐deoxyguanosine (8‐odGsn) and 8‐oxo‐7,8‐dihydroguanosine (8‐oGsn) have potential use in clinical practice. However, biological variation (BV) and reference change values (RCVs) have not been established. The aim of this study was to establish the short‐term between‐subject BV(CV G ) , within‐subject BV(CV I ), and RCVs for urinary 8‐odGsn and 8‐oGsn. Methods First‐morning midstream urine specimens were collected from 20 apparently healthy subjects(ten males and ten females) on five consecutive days. 8‐odGsn and 8‐oGsn were measured using LC‐MS/MS, while urine creatinine (U‐Cr) was also measured to correct their results. A two‐level nested ANOVA was used to estimate the CV I and CV G.Results The values of CV G for 8‐odGsn, 8‐odGsn/U‐Cr, 8‐oGsn, and 8‐oGsn/U‐Cr were 31.2%, 39.6%, 35.3%, and 28.8%, respectively, while CV I for them were 40.5%, 9.0%, 33.5%, and 12.1%, respectively. The RCVs for 8‐odGsn, 8‐odGsn/U‐Cr, 8‐oGsn, and 8‐oGsn/U‐Cr were 112.5%, 26.7%, 93.7%, and 36.5%, respectively. Conclusion BV and RCVs were firstly established for 8‐oxo‐dGsn and 8‐oGsn, and can be used in clinical practice.