Open AccessAnalysis of circular RNA expression profiles of lung cancer in Xuanwei, China
Author(s) -
Wang Yan,
Lu LingJiao,
Duan Yong,
Liu Xiao,
Mao Yue,
Chen Yu,
Zhang YanLiang
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23521
Subject(s) - circular rna , microarray , lung cancer , microbiology and biotechnology , real time polymerase chain reaction , microarray analysis techniques , gene , biology , rna , gene expression , medicine , pathology , genetics
Background Mounting evidence indicates that circular RNAs (circRNAs) could play a pivotal role in cancers. However, due to the lack of sensitive biomarkers, most lung cancer in Xuanwei (LCXW) patients are still diagnosed at an advanced stage accompany with distant metastasis. Methods According to the stage of LCXW patients and tissue sources, circRNAs microarray detection was carried out in six groups. Considering fold change, raw intensity, the length of circRNAs, and P‐ value, we selected eight circRNAs for further study. A total of 50 paired LCXW tissues were carried out real‐time quantitative polymerase chain reaction (RT‐qPCR) in order to extended sample size to verify the expression of these circRNAs. Results We designed 13 617 human circRNA probes for the human circular RNA microarray, detected 10 819 circRNA in six groups of samples; 537 circRNAs were differentially expressed consistently in every stage. Through RT‐qPCR, we selected 8 circRNAs, three of which were upregulated (hsa_circ_0005927, hsa_circ_0069397 and hsa_circ_0000937) and five were downregulated (hsa_circ_0001936, hsa_circ_0005255, hsa_circRNA_406010, hsa_circ_0007064, hsa_circ_0000907) in tumor tissues, only hsa_circ_0001936 showed the opposite expression between microarray and RT‐qPCR, others were consistent. Additionally, hsa_circ_0005927 and hsa_circ_0001936 were significantly correlated with tumor size, and hsa_circRNA_406010 was related to the prognosis of LCXW patients. Conclusion Together, these results suggest that hsa_circ_0005927, hsa_circ_0001936, and hsa_circRNA_406010 may serve as the novel potential biomarkers for LCXW. Moreover, these results may provide a new insight for the pathogenesis of LCXW.