Open AccessAssociation of genetic variants in lncRNA GAS5/miR‐21/mTOR axis with risk and prognosis of coronary artery disease among a Chinese population
Author(s) -
Li Hu,
Liu Yingxue,
Huang Jinyan,
Liu Yu,
Zhu Yufeng
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23430
Subject(s) - single nucleotide polymorphism , gas5 , multifactor dimensionality reduction , medicine , snp , coronary artery disease , oncology , bioinformatics , genotype , genetics , biology , long non coding rna , gene , rna
Background Allowing for the significance of single nucleotide polymorphisms (SNPs) in reflecting disease risk, this investigation attempted to uncover whether SNPs situated in lncRNA GAS5/miR‐21/mTOR axis were associated with risk and prognosis of coronary heart disease (CHD) among a Chinese Han population. Methods Altogether 436 patients with CHD were recruited as cases, and meanwhile, 471 healthy volunteers were included into the control group. Besides, SNPs of GAS5 /MIR‐21/ mTOR axis were genotyped utilizing mass spectrometry. Chi‐square test was applied to figure out SNPs that were strongly associated with CHD risk and prognosis, and combined effects of SNPs and environmental parameters on CHD risk were evaluated through multifactor dimensionality reduction (MDR) model. Results Single nucleotide polymorphisms of GAS5 (ie, rs2067079 and rs6790), MIR‐21 (ie, rs1292037), and mTOR (rs2295080, rs2536, and rs1034528) were associated with susceptibility to CHD, and also Gensini score change of patients with CHD ( P < .05). MDR results further demonstrated that rs2067079 and rs2536 were strongly interactive in elevating CHD risk ( P < .05), while smoking, rs6790 and rs2295080 showed powerful reciprocity in predicting Gensini score change of patients with CHD ( P < .05). Conclusion Single nucleotide polymorphisms of lncRNA GAS5/miR‐21/mTOR axis might interact with smoking to regulate CHD risk, which was conducive to diagnosis and prognostic anticipation of CHD.