Open AccessHypermethylation of lncRNA MEG3 impairs chemosensitivity of breast cancer cells
Author(s) -
Li Hongchang,
Wang Puhua,
Liu Jiazhe,
Liu Weiyan,
Wu Xubo,
Ding Junbin,
Kang Jie,
Li Jindong,
Lu Jingfeng,
Pan Gaofeng
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23369
Subject(s) - meg3 , cell culture , cancer research , methylation , deoxycytidine , cell growth , paclitaxel , cell , metastasis , mtt assay , chemistry , biology , cancer , microbiology and biotechnology , medicine , downregulation and upregulation , long non coding rna , gemcitabine , biochemistry , genetics , gene
Background Chemoresistance posed a barrier to successful treatment of breast cancer (BC), and lncRNA MEG3 has been documented to implicate in BC development. However, whether MEG3 methylation, which led to low MEG3 expression, was relevant to BC progression and chemoresistance remained uncertain. Methods In the aggregate, 374 pairs of tumor tissues and adjacent normal tissues were collected from pathologically confirmed BC patients, and four BC cell lines, including MDA‐MB‐231, Bcap‐37, MCF‐7, and SK‐BR‐3, were purchased. Moreover, methylation‐specific polymerase chain reaction (PCR) was adopted to evaluate the methylation status of BC tissues and cell lines, and chemo‐tolerance of BC cell lines was assessed by performing MTT assay. Concurrently, transwell assay and scratch assay were carried out to estimate the migratory and invasive capability of BC cell lines. Results Methylated MEG3, lowly expressed MEG3, large tumor size (≥2 cm), advanced TNM grade and lymphatic metastasis were potentially symbolic of poor prognosis among BC patients ( P < .05). Besides, MDA‐MB‐231 cell line exhibited the strongest resistance against paclitaxel, adriamycin, and vinorelbine ( P < .05), while MCF‐7 cell line seemed more sensitive against these drugs than any other BC cell line ( P < .05). Furthermore, pcDNA3.1‐MEG3 and 5‐Aza‐dC markedly sensitized MDA‐MB‐231 and MCF‐7 cell lines against the drug treatments ( P < .05). Simultaneously, proliferation and metastasis of the BC cell lines were slowed down under the force of pcDNA3.1‐MEG3 and 5‐Aza‐dC ( P < .05). Conclusion Preventing methylation of MEG3 might matter in lessening BC chemoresistance, owing to its hindering proliferation and metastasis of BC cells.