Open AccessHsa_circ_0012563 promotes migration and invasion of esophageal squamous cell carcinoma by regulating XRCC1/EMT pathway
Author(s) -
Zhang Zhuo,
Li Xueman,
Xiong Fei,
Ren Zhangtao,
Han Yongming
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23308
Subject(s) - gene knockdown , epithelial–mesenchymal transition , cancer research , oncogene , cell migration , cell growth , downregulation and upregulation , carcinogenesis , cell , mtt assay , cell culture , chemistry , apoptosis , microbiology and biotechnology , biology , cell cycle , gene , biochemistry , genetics
Background Recent reports have indicated that circular RNA (circRNA) may regulate tumorigenesis development. However, the function of circRNAs in esophageal squamous cell carcinoma (ESCC) is unclear. Material and method The RT‐qPCR assay was performed to detect hsa_circ_0012563 expression in ESCC tissues and cell lines. Then, the MTT assay, colony formation assay, flow cytometric assay, and cell migration and invasion assay were performed to examine the function of hsa_circ_0012563. In addition, the RT‐PCR and Western blot were used to detect XRCC1 and epithelial‐to‐mesenchymal transition (EMT) related gene expression. Results The RT‐qPCR revealed that the hsa_circ_0012563 expression was remarkably upregulated in ESCC tissue and ESCC cell lines. Functionally, downregulation of hsa_circ_0012563 suppressed cell proliferation, migration, and invasion and promoted cell apoptosis. Mechanically, the knockdown of hsa_circ_0012563 inhibited XRCC1‐mediated EMT pathway to suppress cell migration and invasion. Conclusions Therefore, these results reveal hsa_circ_0012563 is a critical oncogene and may be a novel biomarker in ESCC.