Open AccessCircAP2A2 acts as a ceRNA to participate in infantile hemangiomas progression by sponging miR‐382‐5p via regulating the expression of VEGFA
Author(s) -
Yuan Xiaoqi,
Xu Yanan,
Wei Zhiqiang,
Ding Qi
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23258
Subject(s) - gene knockdown , competing endogenous rna , vascular endothelial growth factor a , cell growth , microrna , mtt assay , western blot , cancer research , biology , downregulation and upregulation , microbiology and biotechnology , cell culture , vegf receptors , vascular endothelial growth factor , gene , genetics , long non coding rna
Background Increasing evidences reveal that circular RNAs (circRNAs) play crucial functions in cancer development. However, the expression pattern and roles of circRNAs in infantile hemangiomas (IH) remain unclear. Methods In this study, qRT‐PCR was performed to determine the expression of circAP2A2, miR‐382‐5p, and VEGFA in IH tissues and cell lines. Moreover, MTT assay, colony formation, transwell assay, and Western blot analysis were conducted to assess the function of circAP2A2 or miR‐382‐5p on cell proliferation, and migration in vitro, respectively. Also, dual luciferase assay was used to confirm the interactions among circAP2A2, miR‐382‐5p, and VEGFA. Results CircAP2A2 was confirmed to be highly expressed in IH. CircAP2A2 knockdown or miR‐382‐5p overexpression decreased the proliferation, colony formation, migration, and invasion of HemECs and HUVEC cells. Conclusion CircAP2A2 could promote proliferation and invasion of IH by regulating miR‐382‐5p/VEGFA axis.