Open AccessDiminished 25‐OH vitamin D 3 levels and vitamin D receptor variants are associated with susceptibility to type 2 diabetes with coronary artery diseases
Author(s) -
Ma Lei,
Wang Shujin,
Chen Heming,
Cui Lin,
Liu Xiaoxiang,
Yang Hua,
Li Guohong,
Liu Songfang,
Qi Ting,
Tian Hongyan
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23137
Subject(s) - foki , taqi , calcitriol receptor , vitamin d and neurology , coronary artery disease , medicine , diabetes mellitus , type 2 diabetes , vitamin d deficiency , endocrinology , genotype , gastroenterology , genetic predisposition , restriction fragment length polymorphism , disease , polymorphism (computer science) , biology , genetics , gene
Background Role of plasma vitamin D and genetic variants of its receptor (VDR) in susceptibility to different diseases has been documented. Various studies in different populations have been highlighted strong associations with diabetes and cardiovascular diseases. Vitamin D deficiency has been linked with the development of type 2 diabetes (T2D) and the onset of coronary artery diseases (CAD). However, the role of vitamin D in predisposition to CAD in patients with T2D is ill‐defined. Materials and Methods We enrolled 674 Chinese T2D patients, and based on clinical phenotype, patients were further categorized into patients with (n = 138) or without coronary artery disease (n = 536). Five hundred twenty‐one healthy subjects from similar geographical areas, free from diabetic or coronary disorders, were enrolled as controls. Serum levels of 25‐OH vitamin D were quantified by ELISA. Common VDR ( FokI , TaqI , BsmI, and ApaI ) polymorphisms were genotyped by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP). Results Patients with T2D displayed lower levels of 25‐OH vitamin D compared with healthy controls. Furthermore, T2D patients with CAD clinical phenotype had the lowest levels of vitamin D. Prevalence of FokI and TaqI mutants was significantly higher in diabetic patients when compared to controls. Interestingly, Tt genotype was more frequent in the artery disease group in comparison with T2D patients without heart involvement. Combined analysis of VDR polymorphisms and serum levels of vitamin D revealed a significant role in predisposition to T2D with or without CAD. Conclusions Lower vitamin D levels and variants of VDR polymorphisms ( FokI and TaqI ) are associated with susceptibility to T2D and clinical manifestation.