Open AccessPrognostic relevance of telomere length and telomerase reverse transcriptase variant (rs2242652) on the multiple myeloma patients
Author(s) -
Aref Salah,
Al Saeed Alshaimaa,
El Menshawy Nadia,
Abdalla Doaa,
El Ashery Mohamed
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23133
Subject(s) - telomere , reverse transcriptase , genotyping , genotype , telomerase reverse transcriptase , multiple myeloma , telomerase , medicine , odds ratio , polymerase chain reaction , gastroenterology , stage (stratigraphy) , oncology , biology , microbiology and biotechnology , genetics , gene , paleontology
Background The search for enhancement of multiple myeloma prognostic tools is an area of current research. This study aimed to assess the clinicopathological impact of telomere length and telomerase reverse transcriptase (TERT) polymorphic variant, rs2242652, on multiple myeloma (MM) patients. Methods Fifty MM patients and 50 healthy controls were included. Relative telomere length (RTL) and rs2242652 genotype polymorphic variants of TERT were analyzed using real‐time polymerase chain reaction (PCR). The MM patients' group was categorized into stage I (n = 16); stage II (n = 12), and stage III (n = 22). Results The median telomere length was significantly longer in MM patients' group (0.78) as compared to controls (0.43) ( P = .001). Multivariate regression analysis revealed that MM patients with RTL < 0.5 had significant poor response for induction remission therapy with odds ratio 26.45. On the other hand, TERT genotyping analysis of rs2242652 revealed insignificant difference between cases and controls ( P = .234), regarding to induction remission response. Survival analysis using Kaplan‐Meier curve revealed that patients with shorter telomere length and those with TERT genotype GA had shorter overall survival. Conclusion Telomere length and TERT rs2242652 genotype polymorphism could be used for refining risk stratification of MM patients.