Open AccessGastric juice piR‐1245: A promising prognostic biomarker for gastric cancer
Author(s) -
Zhou Xiaorong,
Liu Jianhong,
Meng Aifeng,
Zhang Lihong,
Wang Min,
Fan Hong,
Peng Wei,
Lu Jianwei
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23131
Subject(s) - biomarker , cancer , medicine , receiver operating characteristic , gastroenterology , carcinogenesis , stomach , confidence interval , oncology , biology , biochemistry
Background Emerging reports demonstrated that PIWI‐interacting RNAs (piRNAs) played an indispensable role in tumorigenesis. However, it still remains elusive whether piR‐1245 in gastric juice specific in stomach could be employed as a biomarker for gastric cancer (GC). The present work is aiming at exploring the possibility of piR‐1245 in gastric juice as a potential marker to judge for diagnosis and prognosis of gastric cancer. Methods Gastric juice was collected from 66 GC patients and 66 healthy individuals. Quantitative real‐time reverse transcriptase polymerase chain reaction (qRT‐PCR) was employed to measure the levels of piR‐1245 expression. Then, the pattern of piR‐1245 expression in gastric juice was determined between GC patients and healthy individuals. A receiver operating characteristic (ROC) curve was constructed for distinguishing GC from healthy individuals. Results Gastric juice piR‐1245 levels in GC were higher than those of controls ( P < .0001). The value of area under ROC (AUC) was 0.885 (sensitivity, 90.9%; specificity, 74.2%; 95% confidence interval, 0.8286 to 0.9414). High gastric juice piR‐1245 expression was signally correlated with tumor size ( P = .013) and TNM stage ( P = .001). GC patients with high piR‐1245 expression in gastric juice exerted a poorer overall survival (OS) ( P = .0152) and progression‐free survival (PFS) ( P = .013). COX regression analysis verified that gastric juice piR‐1245 expression was an independent prognostic risk variable for OS ( P < .05). Conclusions The current study suggested that piR‐1245 in gastric juice had the potential to be a useful biomarker for GC detection and prognosis prediction.