Open AccessOxidative stress enhances the immune response to oxidatively modified catalase enzyme in patients with Graves’ disease
Author(s) -
Gargouri Bochra,
Mseddi Malek,
Mnif Fatma,
Abid Mohamed,
Attia Hamadi,
Lassoued Saloua
Publication year - 2020
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23051
Subject(s) - autoantibody , oxidative stress , catalase , malondialdehyde , chemistry , enzyme , endocrinology , hydrogen peroxide , antibody , medicine , reactivity (psychology) , immunology , immune system , autoimmune disease , biochemistry , pathology , alternative medicine
Background Oxidative stress is associated with several autoimmune disorders and oxidative modification of proteins that may result in autoimmune response. This study aims to evaluate the catalase (CAT) activity and the autoimmune response against the native CAT and the oxidatively modified enzyme in patients with Graves' disease (GD) and healthy controls in a comparative way. Methods The CAT activity was evaluated via spectrophotometric method. Using enzyme‐linked immunosorbent assay, the reactivities of autoantibody toward native, malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) modified CAT were evaluated in plasmas of patients and controls. Results Reduced CAT activity was found in patients compared with controls ( P < .05). It was proved that levels of IgG antibodies against MDA‐modified CAT were higher than against unmodified ones ( P < .001). No changes were found for the reactivities to H 2 O 2 ‐modified CAT. Positive correlation was found between the reactivity to MDA‐modified CAT and the triiodothyronine level ( P < .001, r = .6). Conclusion Our findings incriminate the MDA in the autoantibodies reactivity to oxidatively modified CAT leading to a disturbed oxidative profile and/or the progression of GD pathology.