Open AccessComparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens
Author(s) -
Huh Hee Jae,
Ki ChangSeok,
Yun Sun Ae,
Lee Jungsoo,
Oh Gwi Young,
Lee NamSihk,
Yoon Young Ho,
Lee Nam Yong
Publication year - 2019
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22703
Subject(s) - mycoplasma genitalium , ureaplasma urealyticum , chlamydia trachomatis , multiplex , mycoplasma hominis , trichomonas vaginalis , neisseria gonorrhoeae , sexually transmitted disease , virology , microbiology and biotechnology , mycoplasma , ureaplasma , medicine , multiplex polymerase chain reaction , biology , polymerase chain reaction , syphilis , gene , bioinformatics , human immunodeficiency virus (hiv) , biochemistry
Background The DiaPlexQ™ STI6 Detection Kit (DiaPlexQ; Solgent Co., Ltd., Daejeon, South Korea) is a multiplex real‐time PCR assay for the detection of the following sexually transmitted disease (STD) pathogens: Chlamydia trachomatis , Neisseria gonorrhoeae , Mycoplasma hominis , Trichomonas vaginalis , Ureaplasma urealyticum , and Mycoplasma genitalium . We compared the performance of the DiaPlexQ assay with the GeneFinder™ STD I (CT/NG/UU) and STD II (MG/MH/TV) Multiplex Real‐time PCR Kits (GeneFinder; Infopia Co., Ltd., Anyang, South Korea). Methods We evaluated the performance of the DiaPlexQ assay in comparison to that of GeneFinder using 1106 clinical specimens (542 genital swabs and 564 urine samples). The analytical performance of the DiaPlexQ assay, including the limit of detection (LOD) and analytical specificity, was evaluated using reference strains. Results The positive percent agreement, negative percent agreement, and kappa value between the two assays were 96.6%‐99.4%, 98.2%‐99.8%, and 0.93%‐0.99%, respectively. No cross‐reactivity was observed in a collection of 41 different microorganisms and the LOD of the DiaPlexQ assay ranged from 1 to 10 copies/reaction for each microorganism. Conclusion The DiaPlexQ assay showed comparable performance to that of the GeneFinder assay so that it can be used for the screening and diagnosis of non‐viral curable STD pathogens.