Open AccessAssociation of CD 28 and CTLA 4 haplotypes with susceptibility to primary Sjögren′s syndrome in Mexican population
Author(s) -
LópezVillalobos Erika Fabiola,
CarrilloBallesteros Francisco Josué,
MuñozValle José Francisco,
PalafoxSánchez Claudia Azucena,
Valle Yeminia,
OrozcoBarocio Gerardo,
OregonRomero Edith
Publication year - 2019
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22620
Subject(s) - haplotype , genotype , ctla 4 , immunology , gastroenterology , population , t cell , medicine , microbiology and biotechnology , biology , genetics , gene , immune system , environmental health
Background Primary Sjögren's syndrome ( pSS ) is an autoimmune disease characterized by destruction of exocrine glands as a result of T and B cells infiltrated in glandular tissue. CD 28 and CTLA ‐4 play a crucial role in T cell activation and inhibition. The aim of this study was to associate CD 28 and CTLA 4 haplotypes with susceptibility to pSS in patients from western Mexico. Methods Polymerase chain reaction and restriction fragment length polymorphism were performed to identify CD 28 and CTLA 4 genotypes in 111 patients with pSS and 138 control subjects ( CS ). Haplotype analysis was carried out by SHE sis program. Soluble serum levels of CD 28 ( sCD 28) and CTLA ‐4 ( sCTLA ‐4) were quantified by ELISA kit. Results The CD 28 GC haplotype was associated with low risk to pSS (2.5‐folds, P < 0.001). CTLA 4 CAG and CGA were identified as genetic risk factor ( P < 0.001; OR = 3.82[ CI 95%:2.022‐7.296] and P < 0.001; OR = 11.38[ CI 95%:3.282‐37.69] respectively). No difference in sCD 28 and sCTLA ‐4 were found between patients and CS . However, pSS patients carriers of CD 28 IVS 3 + 17 TC genotype showed high sCD 28 ( P = 0.039 vs TT carriers in CS ). In regard to sCTLA ‐4, patient who carry CTLA 4 ‐319C>T, +49 A>G, and +6230 G>A, or their haplotypes did not show any difference. Conclusion Our findings suggest that CD 28 GC , CTLA 4 CAG , and CGA haplotypes are associated with susceptibility to pSS in patients from western Mexico. It seems that genetic control of CD 28 and CTLA 4 as well as local immune response in glandular tissue may regulate the impact of the gene expression in pSS . It is necessary to confirm this hypothesis in an integrative study.