Open AccessComparison of whole‐blood tacrolimus concentrations measured by different immunoassay systems
Author(s) -
Kaneko Tetsuya,
Fujioka Takashi,
Suzuki Yosuke,
Nagano Toshiaki,
Sato Yuhki,
Asakura Syunji,
Itoh Hiroki
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22587
Subject(s) - immunoassay , chromatography , chemiluminescent immunoassay , chemistry , tacrolimus , coefficient of variation , whole blood , correlation coefficient , liquid chromatography–mass spectrometry , mass spectrometry , medicine , mathematics , immunology , antibody , transplantation , statistics
Different measured values for tacrolimus were obtained with different automated immunoassays. We aimed to examine the differences in the blood tacrolimus concentrations measured by the major immunoassay systems commercially available in Japan. Methods Whole‐blood samples from 118 patients were assayed by 3 commercial assays: chemiluminescent enzyme immunoassay (CLIA), affinity column‐mediated immunoassay (ACMIA), and enzyme‐multiplied immunoassay technique (EMIT). Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) was used for reference. Key findings The correlation coefficient of immunoassay vs LC‐MS/MS was excellent for ACMIA (.83) and CLIA (.81) and good for EMIT (.71). The mean error was negative for ACMIA and positive for CLIA and EMIT. The mean absolute error and root‐mean‐square error were almost the same for ACMIA and CLIA and lower than those for EMIT. Conclusions The ACMIA and CLIA yield considerably better results than the EMIT for monitoring blood tacrolimus concentrations.