Open AccessThe first case of congenital blood chimerism in two of the triplets in Korea
Author(s) -
Chung Yoo Na,
Chun Sejong,
Phan MinhTrang Thi,
Nam MyungHyun,
Choi Byung Min,
Cho Duck,
Choi Ji Seon
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22580
Subject(s) - abo blood group system , chimera (genetics) , agglutination (biology) , biology , genotype , blood grouping , microbiology and biotechnology , monoclonal antibody , immunology , polymerase chain reaction , allele , genetics , antibody , gene
Background Chimeras are composed of two or more different populations that originated from different zygotes. Blood chimerism arising from twins have been reported in the literature. Herein, we report the first blood group chimerism in triplets. Methods ABO blood grouping was carried out by manual tile methods (Merck Millipore, UK ) and micro‐column agglutination method (Bio‐Rad, Cressier sur Morat, Switzerland). Flow cytometric analysis was performed with Anti‐A‐ PE conjugated monoclonal antibodies ( BD Biosciences, San Jose, CA , USA ) and FACS Canto II ( BD Biosciences). Molecular analysis was performed with allele‐specific polymerase chain reaction ( AS ‐ PCR ) and direct sequencing of the exons 6 and 7. Results Mixed‐field agglutination and weak agglutination against anti‐A were revealed by ABO blood grouping. Flow cytometric analysis revealed the presence of both A cells and O cells. AS ‐ PCR and sequencing showed two neonates with chimerism, with each neonate`s genotype being A102/O01/O02 . Conclusion This is the first recorded case of blood chimera from a triplet in Korea. We recommend full investigation of blood group chimerism in neonates with ABO discrepancy, as blood chimerism is subject to certain caution in the clinical environment.