Open AccessAutomated analysis for differentiating leukocytes in body fluids using the software “biological liquid application” on ADVIA 2120/2120i hematology analyzer
Author(s) -
Falda Alessandra,
Doretto Paolo
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22578
Subject(s) - hematology analyzer , body fluid , repeatability , peritoneal dialysis , pathology , pleural fluid , biomedical engineering , spectrum analyzer , medicine , chromatography , computer science , chemistry , pleural effusion , telecommunications
We evaluated the “Biological liquid application ADVIA 2120” software for differentiating the percentage of polymorphonucleated (% PMN ) and mononucleated cells (% MN ) in ascitic, pleural, and peritoneal dialysis ( PD ) fluid. Methods Biological fluid test results of 193 specimens obtained by automated methods (87 with and 106 without dedicated software) were compared with May‐Grünwald‐Giemsa ( MGG ) stained blood smears. Limit of detection (LoD) and quantitation (LoQ), repeatability, and inaccuracy were assessed. Results Good agreement between the automated methods with dedicated software and the manual method for % PMN and % MN was obtained for leukocyte differentiation in ascitic and pleural fluids, while correlation with the manual method for PD fluid was poor, both with and without the dedicated software. Conclusions We demonstrated that the automated differentiation of leukocytes with dedicated software on the ADVIA 2120 analyzer for body fluids is a good alternative to the microscopic reference method for peritoneal and pleural specimens, but not for PD fluids.