Open AccessDetermination of antibiotic resistance and high‐performance liquid chromatography profiles for Mycobacterium species
Author(s) -
Toka Özer Türkan,
Yula Erkan,
Doğan Metin,
Baskın Hüseyin
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22459
Subject(s) - mycolic acid , dendrogram , high performance liquid chromatography , mycobacterium tuberculosis , mycobacterium , chromatography , mycobacterium fortuitum , tuberculosis , microbiology and biotechnology , biology , antibiotics , chemistry , medicine , pathology , population , environmental health , genetic diversity
Background Incidence of mycobacterial infections has been increasing. However, diagnosis and treatment of mycobacterial infections can be difficult. The aim of this study was to investigate high‐performance liquid chromatography ( HPLC ) analysis of the mycolic acids for rapid identification and dendrogram cluster analysis of mycobacterium species. Methods Clinical specimens received for mycobacterial culture and antimicrobial susceptibility test were processed by standard laboratory protocols. Positive cultures were analyzed with HPLC method. Mycolic acid analysis with HPLC was used for diagnosis of tuberculosis and other mycobacterial infections. These reports were compared with Sherlock Library mycobacterial species, and the similarity index was analyzed. This value was formed by a software in multidimensional space that was the calculation of the average distance between the nearest library profile and unknown profile. Results The ninety‐two samples were identified as M. tuberculosis . (similarity index between 0.593 and 0.994). One of the other strains was identified as M. avium intracellulare (strain No. 82) ( SI = 0.906); one of them was identified as M. interjectum (strain no. 89) ( SI = 0.644). Total 94 samples were identified, and dendrogram was applied to these samples. Profile A (10.6%), profile B (59.6%), profile C (11.7%), profile D (3.2%), and other profiles as single different profiles were identified. Rates for each as 1% (89, 94, 1, 82, 26, 42, 32, 41, 100, 43, 47, 44, 40, 35). Conclusion High‐performance liquid chromatography is a useful, rapid, reliable, and practical method for diagnosis of mycobacterium species.