Open AccessCircular RNA hsa_circ_0008344 regulates glioblastoma cell proliferation, migration, invasion, and apoptosis
Author(s) -
Zhou Jinxu,
Wang Hongxiang,
Chu Junsheng,
Huang Qilin,
Li Guangxu,
Yan Yong,
Xu Tao,
Chen Juxiang,
Wang Yuhai
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22454
Subject(s) - downregulation and upregulation , gene knockdown , rna , glioblastoma , cell growth , circular rna , microbiology and biotechnology , apoptosis , cancer research , biology , microarray analysis techniques , cell , microarray , chemistry , gene expression , gene , biochemistry
Background Recent studies have found circular RNA s (circ RNA s) involved in the biological process of cancers. However, little is known about their functional roles in glioblastoma. Methods Human circ RNA microarray analysis was performed to screen the expression profile of circ RNA s in IDH 1 wild‐type glioblastoma tissue. The expression of hsa_circ_0008344 in glioblastoma and normal brain samples was quantified by qRT ‐ PCR . Functional experiments were performed to investigate the biological functions of hsa_circ_0008344, including MTT assay, colony formation assay, transwell assay, and cell apoptosis assay. Results Circ RNA microarray revealed a total of 417 abnormally expressed circ RNA s (>1.5‐fold, P < .05) in glioblastoma tissue compared with the adjacent normal brain. Hsa_circ_0008344, among the top differentially expressed circ RNA s, was significantly upregulated in IDH 1 wild‐type glioblastoma. Further in vitro studies showed that knockdown of hsa_circ_0008344 suppressed glioblastoma cell proliferation, colony formation, migration, and invasion, but increased cell apoptotic rate. Conclusions Hsa_circ_0008344 is upregulated in glioblastoma and may contribute to the progression of this malignancy.