Open AccessModified Carba NP Test: Simple and rapid method to differentiate KPC‐ and MBL‐producing Klebsiella species
Author(s) -
Kumar Nitin,
Singh Varsha A.,
Beniwal Vikas,
Pottathil Shinu
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22448
Subject(s) - klebsiella pneumoniae , predictive value , klebsiella , gold standard (test) , polymerase chain reaction , biology , microbiology and biotechnology , medicine , genetics , escherichia coli , gene
Background The aim of this study was to evaluate the modified Carba NP test to differentiate KPC ( Klebsiella pneumoniae carbapenemase)‐ and MBL (metallo‐β‐lactamase)‐producing Klebsiella species. Methods A total of 508 non‐duplicate clinical isolates of Klebsiella spp. were processed by modified Carba NP and combined disc tests which were further confirmed by conventional polymerase chain reaction (PCR), a gold standard method for statistical analysis. Results Modified Carba NP test demonstrated 91.7% sensitivity, 100% specificity, 100% positive predictive value (PPV) and 99.8% negative predictive value (NPV) for KPC and 96.7%, 100%, 100%, and 99.5% for MBL detection, respectively. Conclusion The performance of modified Carba NP test was significantly better than combined disc test, fulfilling the requirement of simple and rapid test for clinical applications.