Open AccessAnalysis of SLCO 1B1 and APOE genetic polymorphisms in a large ethnic Hakka population in southern China
Author(s) -
Zhong Zhixiong,
Wu Heming,
Li Bin,
Li Cunren,
Liu Zhidong,
Yang Min,
Zhang Qifeng,
Zhong Wei,
Zhao Pingsen
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22408
Subject(s) - ethnic group , china , chinese population , southern china , polymorphism (computer science) , population , genetics , biology , genotype , gene , geography , medicine , political science , environmental health , archaeology , law
Objective Statins are the most widely used lipid‐lowering drugs, which have a significant effect on the inhibition of cardiovascular disease. The efficacy and side effects of statins are associated with the polymorphisms of SLCO 1B1 and APOE genes. The purpose of this study was to analyze the SLCO 1B1 and APOE gene polymorphisms in the Hakka population of southern China. Methods A total of 3249 subjects including 2019 males and 1230 females participated in this study. Polymerase chain reaction ( PCR )‐fluorescence probe technique for polymorphisms analysis and analyzed the genotypes frequencies of SLCO 1B1 and APOE genes. Results The frequencies of SLCO 1B1 521T>C between men and women were statistically significant ( SLCO 1B1 521 TT , χ 2 = 8.431, P = .004; SLCO 1B1 521 TC , χ 2 = 7.436, P = .007). The frequencies of haplotypes *1b/*1b (40.07%) and *1a/*1b (32.56%) of SLCO 1B1 gene accounted for 72.63%, followed by *1b/*15(14.40%), *1a/*1a (5.82%), *1a/*15 (5.57%), *15/*15 (1.45%), and *1a/*5 (0.12%). The frequencies of haplotypes *1a/*15 and *1b/*1b of SLCO 1B1 gene between men and women were statistically significant (*1a/*15, χ 2 = 6.789, P = .009; *1b/*1b, χ 2 = 3.998, P = .004). In this study, genotype ɛ3/ɛ3 accounted for 69.04%, followed by ɛ3/ɛ4 (16.19%), ɛ2/ɛ3 (11.60%), ɛ2/ɛ4 (1.35%), ɛ4/ɛ4 (1.08%), and ɛ2/ɛ2 (0.74%) in all subjects, in which ɛ3 had the greatest allele frequency (82.93%), followed by ɛ4 (9.85%) and ɛ2 (7.22%). We found that 47 subjects carrying the SLCO 1B1 521 ( CC ) polymorphism who had not any myopathy caused by statins. Conclusions We analyzed the SLCO 1B1 and APOE gene polymorphisms in the Hakka population of southern China. This study provides a reference for the individualized meditation for Hakka population in this area.