Open AccessGenetic and phenotypic characterizations of drug‐resistant Mycobacterium tuberculosis isolates in Cheonan, Korea
Author(s) -
Jeon JaeSik,
Kim Jae Kyung,
Choi Qute,
Kim Jong Wan
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22404
Subject(s) - rpob , inha , mycobacterium tuberculosis , tuberculosis , biology , genotyping , drug resistance , microbiology and biotechnology , antibiotics , gene , genetics , virology , genotype , medicine , pathology
Background Mycobacterium tuberculosis ( MTB ) causes tuberculosis ( TB ), which is a fatal disease. Cases of drug‐resistant MTB have increased in recent years. In this study, we analyzed 7 sites of MTB DNA sequences, including the rpoB and inhA gene, to investigate the relationship between gene mutations and drug resistance in MTB . Methods Mycobacterium tuberculosis liquid culture samples (197 specimens from 74 cases) were collected between June 2015 and May 2016 and sequenced. The results were compared with those obtained from antibiotic susceptibility tests. Results In 65 (87.8%) cases, the antibiotic‐resistant phenotype was consistent with genotyping results, whereas in 9 (12.2%) cases, there was no match. Eight mutations were detected in the rpoB gene, which showed the highest mutation rate. Sequencing results indicated that these mutations were present in 12 cases. Conclusion Previously published data on antibiotic resistance genes are insufficient for effective prevention of multidrug‐ or extensive drug‐resistant TB . Additional studies are needed to characterize the complement of antibiotic resistance genes in MTB .