Open AccessDiagnostic validity of flow cytometry vs manual counting of polymorphonuclear leukocytes in spontaneous bacterial peritonitis
Author(s) -
Osman Heba Ahmed,
Aly Sanaa Shaker,
SalahEldin Eman M.,
ElMasry Muhammad Abbas,
Hassan Mohammed H.
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22395
Subject(s) - spontaneous bacterial peritonitis , flow cytometry , medicine , ascitic fluid , cirrhosis , cell counting , pathology , gastroenterology , ascites , cytometry , peritonitis , immunology , cancer , cell cycle
Background Spontaneous bacterial peritonitis ( SBP ) is frequently occurring infection among patients with liver cirrhosis, defined by polymorphonuclear ( PMN ) leukocytic count ≥250 cell/mm 3 with or without a positive ascitic fluid ( AF ) bacterial culture . So, this study aimed to investigate the diagnostic value of flow cytometry versus manual counting of ascitic fluid PMNL in cirrhotic patients, with clinical suspicion of SBP . Methods A hospital‐based cross‐sectional study was carried out on 320 cirrhotic patients with clinical suspicion of SBP . Abdominal paracentesis was performed in all cases for microscopic manual and flow cytometry counting of PMNL . Anti‐ HLA ‐ DR , anti‐ CD 15, anti‐ CD 16, and anti‐ CD 45 monoclonal antibodies were used for flow cytometry method. Results Flow cytometric PMNL count had 100% sensitivity and specificity, while manual PMNL count had a sensitivity of 65.52% and specificity of 90% with significant difference ( P value < .05). Conclusion Flow cytometry is more reliable rapid method for PMNL counting, than the manual method that is less accurate and time‐consuming in diagnosing clinically suspected SBP.