Open AccessGenetic analysis of interleukin 18 gene polymorphisms in alopecia areata
Author(s) -
Celik Sumeyya Deniz,
Ates Omer
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22386
Subject(s) - alopecia areata , single nucleotide polymorphism , genotype , snp , genotyping , allele , interleukin 18 , allele frequency , polymorphism (computer science) , medicine , immunology , biology , genetics , gene , cytokine
Background Alopecia areata ( AA ), which appears as nonscarring hair shedding on any hair‐bearing area, is a common organ‐specific autoimmune condition. Cytokines have important roles in the development of AA . Interleukin ( IL ) 18 is a significant proinflammatory cytokine that was found higher in the patients with AA . We aimed to investigate whether the IL ‐18 (rs187238 and rs1946518) single nucleotide polymorphisms ( SNP s) may be associated with AA and/or clinical outcome of patients with AA in Turkish population. Methods Genotyping of rs187238 and rs1946518 SNP s were detected using sequence‐specific primer‐polymerase chain reaction ( SSP ‐ PCR ) method in 200 patients with AA and 200 control subjects. Results The genotype distribution of rs1946518 (−607C>A) SNP was found to be statistically significantly different among patients with AA and controls ( P = .0008). Distribution of CC + CA genotypes and frequency of −607/allele C of rs1946518 SNP were higher in patients with AA ( P = .001, P = .001, respectively). The genotype distribution of rs187238 (−137G>C) SNP was found to be statistically significantly different among patients with AA and control subjects ( P = .0014). Distribution of GG genotype and frequency of −137/allele G of rs187238 SNP were higher in patients with AA ( P = .0003, P = .001, respectively). Conclusion The rs1946518 (−607C>A) and rs187238 (−137G>C) polymorphisms were found associated with alopecia areata disease. The study suggests that IL ‐18 rs187238 and rs1946518 SNP s may be the cause of the AA susceptibility.