Circular RNA hsa_circ_0000567 can be used as a promising diagnostic biomarker for human colorectal cancer

Background Recent studies have revealed that circular RNA s are involved in the biological process of some kinds of human cancers. However, little is known about their diagnostic values and functions in colorectal cancer ( CRC ). Methods The expression levels of hsa_circ_0000567 in 102 paired CRC tissues and adjacent noncancerous tissues, 5 CRC cell lines, and a normal colorectal epithelial cell line were detected by quantitative real‐time polymerase chain reaction ( qRT ‐ PCR ). The correlations between hsa_circ_0000567 expression levels and the clinicopathological factors of patients with CRC were analyzed. Furthermore, the loss‐of‐function assay was performed to investigate the functions of hsa_circ_0000567 in vitro. Finally, a receiver operating characteristic ( ROC ) curve was established to evaluate the diagnostic value of hsa_circ_0000567. Results Hsa_circ_0000567 expression was significantly downregulated in CRC tissues and CRC cell lines. In addition, the decreased hsa_circ_0000567 expression in CRC was negatively correlated with tumor size ( P  =   .011), lymph metastasis ( P  =   .003), distal metastasis ( P  <   .0001), and tumor–node–metastasis ( TNM ) stage ( P  =   .003) in CRC . Moreover, knockdown of hsa_circ_0000567 promoted CRC cells proliferation and migration in vitro. Importantly, the area under the ROC curve ( AUC ) was 0.8653, which indicates hsa_circ_0000567 can serve as a diagnostic biomarker. Conclusion Hsa_circ_0000567 may be a novel suppressor and a potential diagnosis biomarker in CRC .