Open AccessThe correlation between SNP s within the gene of adrenergic receptor and neuropeptide Y and risk of cervical vertigo
Author(s) -
Han Jianlong,
Zuo Jinliang,
Zhu Dengsong,
Gao Chunzheng
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22366
Subject(s) - snp , vertigo , single nucleotide polymorphism , genotyping , taqman , haplotype , medicine , neuropeptide y receptor , polymerase chain reaction , snp genotyping , genotype , biology , gene , genetics , receptor , surgery , neuropeptide
Background The current investigation was aimed to explore the potential associations of SNP s within ADRB 2 , ADRB 1, NPY , and ADRA 1A with risk and prognosis of cervical vertigo. Methods Altogether 216 patients with cervical vertigo and 204 healthy controls were gathered, and their DNA s were extracted utilizing the whole‐blood DNA extraction kit. Besides, the PCR reactions were conducted using the TaqMan R single nucleotide polymorphism ( SNP ) genotyping assays, and the SNP s were detected on the 7900 HT real‐time fluorogenic quantitative polymerase chain reaction ( PCR ) instrument. Finally, the severity of cervical vertigo was classified according to the JOA scoring, and the recovery rate ( RR ) of cervical vertigo was calculated in light of the formula as:Postoperative JOA − Preoperative JOA 17 − Preoperative JOA× 100 %Results The SNP s within ADRA 1A [rs1048101 (T>C) and rs3802241 (C>T)], NPY [rs16476 (A>C), rs16148 (T>C), and rs5574 (C>T)], ADRB 1 [rs28365031 (A>G)] and ADRB 2 [rs2053044 (A>G)] were all significantly associated with regulated risk of cervical vertigo (all P < .05). Haplotypes of ADRA 1A [ CT and TC ] and NPY [ CCT and ATT ] were also suggested as the susceptible factors of cervical vertigo in comparison with other haplotypes. Furthermore, the SNP s within ADRA 1A [rs1048101 (T>C)], NPY [rs16476 (A>C), rs16148 (T>C)], as well as ADRB 1 [rs28365031 (A>G)] all appeared to predict the prognosis of cervical vertigo in a relatively accurate way (all P < .05). Ultimately, the haplotypes of ADRA 1A ( CC ) and NPY ( CCT ) tended to decrease the RR . Conclusions The SNP s within ADRB 2 , ADRB 1, NPY , and ADRA 1A might act as the diagnostic biomarkers and treatment targets for cervical vertigo.