Open AccessAssociation of GPI a and COX ‐2 gene polymorphism with aspirin resistance
Author(s) -
Wang Hui,
Sun Xiaoxin,
Dong Wanli,
Cai Xiuying,
Zhou Yun,
Zhang Youtao,
Jiang Weijian,
Fang Qi
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22331
Subject(s) - locus (genetics) , genotype , allele , aspirin , gene , gene polymorphism , allele frequency , microbiology and biotechnology , biology , genetics , medicine
Objective This study aimed to explore the association between GPI a, COX ‐2 gene polymorphisms and aspirin resistance in the ischemic stroke patients from the southern part of Jiangsu province. Methods In all, 97 patients with acute ischemic stroke were enrolled in the study. GPI a gene polymorphism at 807C>T (rsl126643) locus and COX ‐2 gene polymorphism at ‐765G>C (rs20417) locus were genotyped by PCR pyrosequencing technology. Patients were divided into the aspirin sensitivity ( AS ) group and aspirin resistance ( AR ) group according to the platelet aggregation rate. The relationship between the two gene polymorphisms and aspirin resistance was investigated and analyzed. Results The distribution of the genotype ( CC , CT , TT , CT + TT , and CC ) and the frequency of allele T of GPI a gene at 807C>T locus were significantly different in AS and AR groups in female patients ( P < .05). Logistic regression analysis showed that the genotype of CT + TT at 807C>T locus was significantly correlated with AR after adjustment for relative factors ( P = .047, OR = 4.856, 95% CI : 1.020–23.108). There were no significant differences in the genotype distribution and allele frequency of the COX ‐2 gene ‐765G>C site between two groups ( P > .05). Conclusion GPI a gene polymorphism at 807C>T locus was associated with AR in Chinese Han females, and the expression of allele T increased the incidence of AR . The gene polymorphism of COX ‐2 gene at ‐765G>C locus was not significantly correlated with AR .