Open AccessRapid detection of the New Delhi metallo‐b‐lactamase 1 ( NDM ‐1) gene by loop‐mediated isothermal amplification ( LAMP )
Author(s) -
Moreira Mirna Giselle,
Barreto Lidia Miranda,
Santos Vera Lúcia,
Monteiro Andrea Souza,
Nobre Vandack,
Santos Simone Gonçalves
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22323
Subject(s) - loop mediated isothermal amplification , klebsiella pneumoniae , gold standard (test) , new delhi , microbiology and biotechnology , polymerase chain reaction , biology , chemistry , gene , virology , medicine , dna , genetics , escherichia coli , metropolitan area , pathology
Background New Delhi Metallo‐b‐lactamase ( NDM ‐1) is an enzyme emerging around the world conferring resistance to a wide range of β‐lactams agents and whose early detection is extremely important. We proposed to standardize the detection of the bla NDM ‐1 gene using the LOOP ‐mediated isothermal amplification technique ( LAMP ). Methods In all, 14 Gram‐negative bacterial strains isolated from patients presenting pneumonia associated with mechanical ventilation were used for the bla NDM ‐1 standardization by LAMP . Klebsiella pneumoniae ATCC BAA ‐2473 and two clinical strains were used as a positive control. All results were compared to the reaction in polymerase chain reaction ( PCR ), considered gold standard for this detection. Results There was an excellent correlation between the two techniques employed, since all measured clinical strains were negative in both employed tests and two clinical, and a reference strains were positive. Conclusions The lamp technique seems to be an excellent option for the rapid detection of bla NDM ‐1. The amplification time is much shorter than other molecular techniques, the PCR machine is not necessary, it is easy of implementation and costs is low.