Open AccessValidation of an automated immune turbidimetric assay for serum gelsolin and its possible clinical utility in sepsis
Author(s) -
HorváthSzalai Zoltán,
Kustán Péter,
Szirmay Balázs,
Lakatos Ágnes,
Christensen Per H.,
Huber Tamás,
Bugyi Beáta,
Mühl Diána,
Ludány Andrea,
Miseta Attila,
Kovács Gábor L.,
Kőszegi Tamás
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22321
Subject(s) - gelsolin , sepsis , western blot , medicine , immunology , immune system , gastroenterology , biology , actin , biochemistry , gene
Background Studies showing the potential predictive value of the actin‐binding protein gelsolin, in critically ill patients are scarce. Moreover, even up to now a rapid automated measurement of gelsolin has still remained a challenge. Therefore, we developed and validated an automated serum gelsolin immune turbidimetric assay for possible clinical use. Methods Validation of serum gelsolin assay was performed on a Cobas 8000/c502 analyzer (Roche) according to the second edition of Eurachem guidelines. Furthermore, we also studied the diagnostic value of serum gelsolin in sepsis when investigating sera of septic (n = 25), systemic inflammatory response syndrome ( SIRS ; n = 8) and control patients (n = 14). We compared our previously published Western blot data with those of the new turbidimetric assay. Results The sample volume was 7 μL and the assay time was 10 minutes. The detection limit was 0.72 mg/L, intra‐ and inter‐assay imprecision remained in most cases less than 5% expressed as CV . Recovery was found to be 84.56%‐93.52% and linearity study gave an appropriate correlation coefficient by linear regression analysis ( r 2 = .998). Septic patients exhibited lower ( P = .015) first‐day serum gelsolin levels than SIRS patients, which confirmed our previous Western blot results. The determined cut‐off point for serum gelsolin was 14.05 mg/L (sensitivity: 75%; specificity: 60%) when investigating its diagnostic value in sepsis. Conclusion Based on the results, our immune turbidimetric measurement offers a rapid and accurate quantitation of gelsolin in human serum samples. Serum gelsolin seems a promising additional diagnostic marker of sepsis which has to be further investigated.