Open AccessCorrelation between TLR 2 , TLR 3 , TLR 4 , and TLR 9 polymorphisms and susceptibility to and prognosis of severe hepatitis among the newborns
Author(s) -
Qiu Xiao,
Dong Yubin,
Cao Yaqin,
Luo Yingmei
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22292
Subject(s) - odds ratio , medicine , gastroenterology , single nucleotide polymorphism , alanine transaminase , immunology , haplotype , hepatitis , biology , genotype , gene , genetics
Background This study was aimed to explore how toll‐like receptor 2 ( TLR 2) , TLR 3 , TLR 4 and TLR 9 influenced the risk and prognosis of severe hepatitis among the Chinese newborns. Methods Altogether 135 newborns diagnosed with severe hepatitis and 140 healthy newborns were included in this study. Totally 12 single nucleotide polymorphisms ( SNP s) within TLR 2 , TLR 3 , TLR 4 , and TLR 9 were chosen and genotyped by polymerase chain reaction‐restriction fragment length polymorphism ( PCR ‐ RFLP ). Odds ratios ( OR s) and 95% confidence intervals ( CI s) were calculated using the logistic regression. The univariate and multivariate analyses were used to analyze independent factors for prognosis of severe hepatitis among the Chinese newborns. Results The SNP s within TLR 2 [ie, rs1898830 (A>G) and rs3804100 (T>C)], TLR 3 [ie, rs1879026 (G>T)], TLR 4 [ie, rs2149356 (T>G)], and TLR 9 [ie, rs187084 (T>C), rs352139 (A>G), and rs352140 (C>T)] were significantly associated with modified risk of neonatal severe hepatitis (all P <.05). Furthermore, rs1898830, rs1879026, rs187084 and rs352139 were also demonstrated to modulate the prognosis [ie, aspartate aminotransferase ( AST )/alanine transaminase ( ALT )>1.5] of newborns with severe hepatitis (all P <.05). Interestingly, the haplotype A‐C‐G‐G‐C‐A‐T were associated with higher susceptibility to neonatal severe hepatitis, and the newborns carrying haplotype A‐C‐G‐G‐C‐A‐T appeared to be correlated with more favorable prognosis (all P <.05). Conclusions Certain SNP s and haplotypes within TLR 2 , TLR 3 , TLR 4 , and TLR 9 can be considered as the potentially treatment targets for neonatal severe hepatitis.