Open AccessPolymorphisms of human leukocyte antigen B*27 on clinical phenotype of spondyloarthritis in Chinese
Author(s) -
Ma HaiJun,
Yin QingFeng,
Liu Yun,
Wu Yin,
Zhu TieChui,
Guo MingHao
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22275
Subject(s) - hla b , genotyping , human leukocyte antigen , subtyping , immunology , pathogenesis , sacroiliitis , polymerase chain reaction , hla b27 , allele , phenotype , group b , biology , medicine , genotype , antigen , genetics , gene , ankylosing spondylitis , computer science , programming language
Background In recent years, an ever‐increasing number of alleles of human leukocyte antigen B*27 ( HLA ‐B*27) have been identified. This study aimed to establish an updated method for HLA ‐B*27 subtyping, and to investigate the impact of HLA ‐B*27 polymorphisms on the clinical phenotype of spondyloarthritis (SpA). Methods Overall, 184 SpA patients were recruited for analyzing diversity of HLA ‐B*27 via an updated high‐resolution polymerase chain reaction amplification with sequence specific primers ( PCR ‐ SSP ). Results The prevalence of HLA ‐B*27 was 94.0%, and four subtypes were identified including HLA ‐B*2704 (77.5%), B*2705 (20.2%), B*2707 (1.7%), and B*2724 (0.6%). There was an obvious male predominance ( P =.05) and markedly elevated C‐reaction protein ( CRP ) in B*27 positive SpA ( P <.01). In multivariate linear regression analysis, the elevated CRP was positively associated with HLA ‐B*27 positivity (regression coefficient B=46.1, P =.0003), grade of sacroiliitis (B=47.5, P =.0032), and male gender (B=20.4, P =.0041). Notably, a male predilection was also found in B*2705 positive SpA while B*2707 was associated with older age, higher positive family history, and higher prevalence of extra‐articular features (all P <.05). Conclusions In this study, an updated PCR ‐ SSP technique to identify increasing alleles of HLA ‐B*27 was developed and their different effects on clinical manifestations of SpA were demonstrated. Genotyping of HLA ‐B*27 would shed light on our understanding of the pathogenesis of SpA.