Open AccessApplication of molecular, microbiological, and immunological tests for the diagnosis of bone and joint tuberculosis
Author(s) -
Tang Yinghua,
Yin Lianli,
Tang Shifu,
Zhang Hongyu,
Lan Jihui
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22260
Subject(s) - medicine , tuberculosis , mycobacterium tuberculosis , rifampicin , immunology , virology , pathology
Background To evaluate the application of interferon gamma release assay ( IGRA ), rifampicin resistant real‐time fluorescence quantitative PCR technique Xpert Mycobacterium tuberculosis /rifampicin (Xpert MTB / RIF ), and the levels of TNF ‐α and TGF ‐β in the diagnosis of bone and joint tuberculosis. Methods Eighty‐six patients with bone and joint tuberculosis, diagnosed by pathology or microbiology, were examined by Xpert MTB / RIF and IGRA (T‐ SPOT . TB ) for Mycobacterium tuberculosis infection, and the TNF ‐α and TGF ‐β levels of the patients were measured. Results The sensitivity of IGRA in diagnosing bone and joint tuberculosis was 81.4%; Xpert MTB / RIF 's sensitivity was 70.9%. The combined sensitivity of the two methods was 91.9%. The combined detection sensitivity of the two methods was higher than individual IGRA or Xpert MTB / RIF detection sensitivity. The TNF ‐α and TGF ‐β levels in bone and joint tuberculosis patients were higher than those in the control group. Conclusion Xpert MTB / RIF , IGRA , TNF ‐α, and TGF ‐βs expression have value in the rapid diagnosis of bone and joint tuberculosis, and the sensitivity and accuracy of bone and joint tuberculosis diagnosis by combining them can improve it.