Open AccessContinuous ambulatory peritoneal dialysis, ascitic and pleural body fluids evaluation with the Mindray BC ‐6800 hematology analyzer
Author(s) -
Fuster Oscar,
Andino Belinda,
Pardo Amparo,
Laiz Begoña
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22240
Subject(s) - hematology analyzer , medicine , continuous ambulatory peritoneal dialysis , detection limit , peritoneal dialysis , hematology , confidence interval , gastroenterology , white blood cell , ambulatory , urology , chromatography , chemistry
Background Accurate evaluation of hematology analyzers is recommended before these devices can be broadly introduced for the routine testing of continuous ambulatory peritoneal dialysis ( CAPD ), ascitic, and pleural fluids. Methods We evaluated the performance of Mindray BC ‐6800 for white blood cell ( WBC ) and differential cell count in 50 CAPD , 60 ascitic and 40 pleural compared with manual microscopy. Within‐run precision, limit of blank (LoB), limit of detection (LoD), limit of quantitation (LoQ), and carryover were assessed. Results The Passing‐Bablok regression in all fluids showed the following equations: y WBC =1.05 x +3.31 (95% CI slope 0.95 to 1.12; intercept −0.25 to 5.52); y MN =0.85 x +15.63 (95% CI slope 0.72 to 1.05; intercept −24.18 to 84.47); and y PMN =1.21 x +13.37 (95% CI slope 1.03 to 1.35; intercept 4.00 to 32.47) with bias 78 cells/μL. The AUC for clinical PMN cut‐off was 0.88 (95% CI : 0.77 to 0.98). In ascitic, pleural, and CAPD fluids the AUC for clinical PMN cut‐off were 0.88 (95% CI : 0.63 to 1.00), 0.83 (95% CI : 0.68 to 0.99), and 1.00 (95% CI : 1.00 to 1.00) respectively. CV ranged from 3%‐34%. LoB of 3 cell/μL was verified. LoD and LoQ reported the same result (8 cells/μL). Carry over never exceeded 0.05%. Conclusion The effectiveness of BC ‐6800 to categorize cells from different body fluids was not compromised by the slight positive bias observed. This conclusion is supported by the high AUC and agreement between the automated method and the reference method. The results show that BC ‐6800 offers rapid, accurate, and reproducible results for clinical management of CAPD , ascitic, and pleural fluids.