Open AccessDifferential expression of urinary exosomal micro RNA s in IgA nephropathy
Author(s) -
Min QingHua,
Chen XiMin,
Zou YeQing,
Zhang Jing,
Li Jing,
Wang Yan,
Li ShuQi,
Gao QiuFang,
Sun Fan,
Liu Jing,
Xu YanMei,
Lin Jin,
Huang LinFeng,
Huang Bo,
Wang XiaoZhong
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22226
Subject(s) - rna , microvesicles , urinary system , exosome , urine , nephropathy , microrna , medicine , real time polymerase chain reaction , rna extraction , antibody , immunology , biology , gene , endocrinology , diabetes mellitus , biochemistry
Background Immunoglobulin A nephropathy (Ig AN ) is the most common type of primary glomerulonephritis in the world. Reliable biomarkers are required for the non‐invasive diagnosis and monitoring of Ig AN . This study aims to investigate the difference in urinary exosomal micro RNA (mi RNA ) expression profiles between patients with IgA nephropathy (Ig AN ) and healthy controls, which may provide clues to identify novel potential non‐invasive mi RNA biomarkers for renal diseases. Methods Urine samples were collected from eighteen healthy controls and eighteen patients with Ig AN . Differential centrifugation was performed to isolate exosomes from urine samples. High‐throughput sequencing and real‐time quantitative polymerase chain reaction ( RT ‐ qPCR ) were sequentially used to screen and further validate mi RNA expression profiles in urinary exosomes of patients with Ig AN in two independent cohorts. Results Urinary exosomes were successfully isolated to obtain exosomal mi RNA s. MiR‐215‐5p and miR‐378i were significantly upregulated in urinary exosomes of patients with Ig AN compared with healthy controls ( P <.01), while miR‐29c and miR‐205‐5p were significantly downregulated ( P <.05). MiR‐215‐5p, miR‐378i, miR‐365b‐3p and miR‐135b‐5p were found to have altered expression in patients with Ig AN from validation cohorts, which was consistent with the high‐throughput sequencing analysis. Conclusion This study suggests that there is a significant difference in urinary exosomal mi RNA profiles between patients with Ig AN and healthy controls. These exosomal mi RNA s, such as miR‐29c, miR‐146a and miR‐205 may potentially serve as novel non‐invasive biomarkers for Ig AN .