Open AccessImpact of micro RNA ‐375 and its target gene SMAD ‐7 polymorphism on susceptibility of colorectal cancer
Author(s) -
Shaker Olfat Gamil,
Mohammed Shereen Rashad,
Mohammed Asmaa Mohammed,
Mahmoud Zeinab
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22215
Subject(s) - colorectal cancer , genotype , medicine , hepatocellular carcinoma , oncology , allele , single nucleotide polymorphism , smad , pathogenesis , gastroenterology , biology , cancer , gene , transforming growth factor , genetics
Background Colorectal cancer ( CRC ) has a high morbidity and mortality. Many studies reported that mir‐375 is frequently down‐regulated in many cancers including esophageal cancer, hepatocellular carcinoma, breast cancer and leukemias. Aim Our aim was to study the expression of micro RNA ‐375 and its target gene SMAD ‐7 polymorphisms (rs4939827) in CRC patients in comparison to control subjects and to correlate these results with clinical data of patients to elucidate their role in pathogenesis and early diagnosis of CRC . Material and methods The present study was conducted on 122 subjects divided into 86 patients with CRC and 36 age‐ and sex‐matched controls. The followings were done to all subjects: full history taking, full clinical examination, complete blood picture, serum ( ALT , AST ), serum albumin, CEA , TLC , PLT , and creatinine. Gene expression of mi RNA ‐375 from serum was done by real‐time PCR . Gene polymorphism SNP s of SMAD 7 (rs4939827) was also done in DNA extracted from blood by real‐time PCR . Results As regards the polymorphism of SMAD 7, we found that CC (wild) genotype has high percentage in controls compared to CRC cases (36.1% vs 15.1%). Meanwhile, the mutant and heterozygotes genotypes showed high percentage among cases compared to controls (33.7%, and 51.2% respectively) vs (22.2%, and 41.7% respectively) with no significant statistical analysis. There was a statistically significant high T‐allelic frequency among cases and C‐allelic frequency among controls. There was a statistically significant association between fold change in micro RNA (‐375) and the susceptibility to CRC as there is down‐regulation of the micro RNA ‐375 in CRC group with fold change in 0.42±0.27. Conclusion Micro RNA ‐375 and rs4939827 SNP in SMAD 7 could be considered as potential markers for detecting and early diagnosing CRC patients.