Open AccessGenotyping of commensal Neisseria spp strains by pulsed‐field gel electrophoresis and 16S rRNA gene sequencing
Author(s) -
Mechergui Arij,
Achour Wafa,
Ben Hassen Assia
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22208
Subject(s) - pulsed field gel electrophoresis , neisseria , 16s ribosomal rna , genotyping , biology , typing , microbiology and biotechnology , ribosomal rna , gene , multilocus sequence typing , genetics , genotype , bacteria
Background We investigated the diversity of the primary sequences of the 16S rRNA genes among 46 commensal Neisseria strains and evaluated the use of this approach as a molecular typing tool in comparison with PFGE analysis. Methods Identification to the genus was done using conventional methods and API NH (bio‐Mérieux ® ). Identification to species level was based on 16S rRNA gene sequencing. PFGE analysis was done using Spe I. Results Fourteen, two, three and fourteen 16S rRNA sequence types were found among twenty Neisseria flavescens , two Neisseria sicca, five Neisseria macacae and nineteen Neisseria mucosa clinical isolates. Forty‐three different PFGE patterns were found among the tested strains. Conclusion We demonstrated a high diversity among 16S rRNA genes which was reflected by PFGE analysis.