Open AccessMolecular characteristics of ofloxacin mono‐resistant Mycobacterium tuberculosis isolates from new and previously treated tuberculosis patients
Author(s) -
Wang Zhirui,
Xie Tong,
Mu Cheng,
Wang Chunhua,
Ju Hanfang,
Zhao Hui,
Sun Rui
Publication year - 2018
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22202
Subject(s) - ofloxacin , mycobacterium tuberculosis , tuberculosis , quinolone , microbiology and biotechnology , dna gyrase , biology , virology , drug resistance , antibiotics , medicine , genetics , gene , ciprofloxacin , escherichia coli , pathology
Background Ofloxacin ( OFX ) resistant Mycobacterium tuberculosis ( MTB ) isolates have been increasingly observed and are a major concern in recent years. This study investigated the genetic mutations associated with OFX resistance among clinical OFX mono‐resistant MTB isolates from new and previously treated tuberculosis patients. Methods A total of 50 unrelated OFX mono‐resistant MTB isolates were analyzed. For all isolates, the quinolone resistance determining regions of gyrA and gyrB were PCR amplified and sequenced. Results Single mutations in the quinolone resistance determining regions of gyrA (positions D94A, G, N, and Y; A90V; and S91P) and gyrB (positions T539A and E540D) were observed in 62% (31/50) and 4% (2/50) of all OFX mono‐resistant isolates, respectively. No differences were detected between the proportions of isolates with mutations in gyrA / gyrB from new and previously treated tuberculosis patients ( P =.820). Conclusions Although mutations in gyrB were rare, they were as important as mutations in gyrA in predicting OFX resistance in MTB in Tianjin, China.