Open AccessPreferential recognition of auto‐antibodies against 4‐ hydroxynonenal modified DNA in the cancer patients
Author(s) -
Faisal Mohammad,
Shahab Uzma,
Alatar Abdulrahman A.,
Ahmad Saheem
Publication year - 2017
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22130
Subject(s) - 4 hydroxynonenal , dna , dna damage , carcinogenesis , epitope , chemistry , biochemistry , mutagenesis , antibody , reactive oxygen species , microbiology and biotechnology , hyperchromicity , lipid peroxidation , biology , oxidative stress , mutation , gene , immunology
Background The structural perturbations in DNA molecule may be caused by a break in a strand, a missing base from the backbone, or a chemically changed base. These alterations in DNA that occurs naturally can result from metabolic or hydrolytic processes. DNA damage plays a major role in the mutagenesis, carcinogenesis, aging and various other patho‐physiological conditions. DNA damage can be induced through hydrolysis, exposure to reactive oxygen species ( ROS ) and other reactive carbonyl metabolites including 4‐hydroxynonenal ( HNE ). 4‐ HNE is an important lipid peroxidation product which has been implicated in the mutagenesis and carcinogenesis processes. Methods The present study examines to probe the presence of auto‐antibodies against 4‐hydroxynonenal damaged DNA ( HNE – DNA ) in various cancer subjects. In this study, the purified calf thymus DNA was damaged by the action of 4‐ HNE . The DNA was incubated with 4‐ HNE for 24 h at 37°C temperature. The binding characteristics of cancer auto‐antibodies were assessed by direct binding and competitive inhibition ELISA . Results DNA modifications produced hyperchromicity in UV spectrum and decreased fluorescence intensity. Cancer sera exhibited enhanced binding with the 4‐ HNE modified calf thymus DNA as compared to its native conformer. The 4‐ HNE modified DNA presents unique epitopes which may be one of the factors for the auto‐antibody induction in cancer patients. Conclusion The HNE modified DNA presents unique epitopes which may be one of the factors for the autoantibody induction in cancer patients.