Open AccessRole of Runx2 polymorphisms in risk and prognosis of ossification of posterior longitudinal ligament
Author(s) -
Chang Feng,
Li Lijun,
Gao Gang,
Ding Shengqiang,
Yang Jincai,
Zhang Ting,
Zuo Genle
Publication year - 2017
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22068
Subject(s) - single nucleotide polymorphism , ossification of the posterior longitudinal ligament , genotype , medicine , allele , gastroenterology , laminoplasty , polymorphism (computer science) , surgery , genetics , biology , gene , myelopathy , spinal cord , psychiatry
Background Our study was aimed at finding out if Runx2 SNP s (single‐nucleotide polymorphisms) are related to susceptibility to and prognosis of ossification of posterior longitudinal ligament ( OPLL ). Methods We selected 80 OPLL patients and another 80 independent patients without OPLL from September 2013 to November 2014. Serum was collected to detect the genotypes of rs1321075, rs12333172, and rs1406846 on Runx2 with direct sequencing analysis. Results Differences in clinical characteristics, including age, weight, height, sex ratio, as well as smoking and drinking history, between OPLL and control groups appeared to be insignificant (all P ‐value >.05). The allele of rs1406846 (A) emerged as a key element in raising OPLL risk with the biggest statistical significance ( P <.001). Conversely, alleles of rs967588 (T) and rs16873379 (C) were associated with reduced predisposition to OPLL less remarkably (both P =.033). Regarding rs16873379, the case group exhibited a smaller frequency of homozygote CC in comparison with TT genotype than the control group ( P =.016). Furthermore, the improvement rate based on calculation of JOA score suggested that genotype AA of rs6908650 was beneficial for OPLL patients' recovery from posterior laminoplasty surgery ( P <.05), while genotypes of rs16873379 ( CC ), rs1406846 ( AA ), and rs2677108 ( CC ) significantly restrained this process ( P <.05). Besides, rs16873379, rs1406846, and rs2677108 were significantly associated with number of ossification segments ( P <.05). Conclusions Runx2 SNP s (e.g., rs16873379, rs1406846, and rs2677108) were strongly correlated with onset and treatment efficacy of OPLL , and they might regulate severity of OPLL .