Open AccessPCR Assay Based on the gyr B Gene for Rapid Identification of Acinetobacter baumannii‐calcoaceticus Complex at Specie Level
Author(s) -
Teixeira Aline B.,
Barin Juliana,
Hermes Djuli M.,
Barth Afonso L.,
Martins Andreza F.
Publication year - 2017
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22046
Subject(s) - acinetobacter baumannii , microbiology and biotechnology , biology , acinetobacter , acinetobacter calcoaceticus , multiplex polymerase chain reaction , polymerase chain reaction , gene , genetics , bacteria , pseudomonas aeruginosa , antibiotics
Background The genus Acinetobacter sp. comprises more than 50 species, and four are closely related and difficult to be distinguished by either phenotypic or genotypic methods: the Acinetobacter calcoaceticus‐baumannii complex (ABC). The correct identification at species level is necessary mainly due to the epidemiological aspects. Methods We evaluated a multiplex PCR for gyr B gene to identify the species of the ABC using the sequencing of the ITS 16S‐23S fragment as a gold standard. Isolates identified as Acinetobacter calcoaceticus‐baumannii from three hospitals at southern Brazil in 2011 were included in this study. Results A total of 117 isolates were obtained and 106 (90.6%) were confirmed as A. baumannii , 6 (5.1%) as A. nosocomialis and 4 (3.4%) as A. pittii by PCR for gyr B gene. Only one isolate did not present a product of the PCR for the gyr B gene; this isolate was identified as Acinetobacter genospecie 10 by sequencing of ITS. We also noted that the non‐ A . baumannii isolates were recovered from respiratory tract (8/72.7%), blood (2/18.2%) and urine (1/9.1%), suggesting that these species can cause serious infection. Conclusion These findings evidenced that the multiplex PCR of the gyr B is a feasible and simple method to identify isolates of the ABC at the species level.