Open AccessDetermination of Urine Albumin by New Simple High‐Performance Liquid Chromatography Method
Author(s) -
Klapkova Eva,
Fortova Magdalena,
Prusa Richard,
Moravcova Libuse,
Kotaska Karel
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.22007
Subject(s) - chromatography , urine , simple (philosophy) , albumin , high performance liquid chromatography , chemistry , biochemistry , philosophy , epistemology
Background A simple high‐performance liquid chromatography ( HPLC ) method was developed for the determination of albumin in patients' urine samples without coeluting proteins and was compared with the immunoturbidimetric determination of albumin. Urine albumin is important biomarker in diabetic patients, but part of it is immuno‐nonreactive. Methods Albumin was determined by high‐performance liquid chromatography ( HPLC ), UV detection at 280 nm, Zorbax 300 SB ‐C3 column. Immunoturbidimetric analysis was performed using commercial kit on automatic biochemistry analyzer COBAS INTEGRA ® 400, Roche Diagnostics GmbH, Manheim, Germany. Results The HLPC method was fully validated. No significant interference with other proteins (transferrin, α‐1‐acid glycoprotein, α‐1‐antichymotrypsin, antitrypsin, hemopexin) was found. The results from 301 urine samples were compared with immunochemical determination. We found a statistically significant difference between these methods ( P = 0.0001, Mann–Whitney test). Conclusion New simple HPLC method was developed for the determination of urine albumin without coeluting proteins. Our data indicate that the HPLC method is highly specific and more sensitive than immunoturbidimetry.