Open AccessGenetic Variants in the Vicinity of LGALS ‐3 Gene and LGALS ‐3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study
Author(s) -
Djordjevic Ana,
Zivkovic Maja,
Stankovic Aleksandra,
Zivotic Ivan,
Koncar Igor,
Davidovic Lazar,
Alavantic Dragan,
Djuric Tamara
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21996
Subject(s) - carotid endarterectomy , genotype , allele , messenger rna , gene expression , gene , snp , phenotype , medicine , haplotype , biology , microbiology and biotechnology , genetics , pathology , bioinformatics , single nucleotide polymorphism , carotid arteries
Background Previous research has shown that there is an association between galectin‐3 (gal‐3) protein and cardiovascular pathology. The aim of this study was to investigate the effects of rs2274273 and rs17128183 on genetic susceptibility to advanced carotid atherosclerosis ( CA ) and its complications. The rs2274273 has been singled out as the lead SNP of the haplotype block containing LGALS ‐3 (gal‐3 gene) associated with gal‐3 circulating levels, while rs17128183 constitutes a potentially functional SNP of the same hap‐block. We further sought to determine whether these genetic variants have an impact on the expression of LGALS ‐3 mRNA in human carotid atherosclerotic plaque tissue. Methods The study encompassed 300 control subjects and 485 patients with advanced CA who had undergone carotid endarterectomy. Rs2274273, rs17128183, and LGALS ‐3 relative mRNA expression was detected by means of real‐time PCR (TaqMan ® technology). Results There were no statistically significant associations of the investigated genetic variants with susceptibility to advanced CA , nor did we find any associations in terms of ultrasonographically defined plaque phenotypes. The relative expression of LGALS ‐3 mRNA proved to be significantly higher in carriers of the rare alleles ( P = 0.039) for both genetic variants. Conclusion Our exploratory results suggest that while rs2274273 and rs17128183 bear no association with the risk of advanced CA or CA ‐related complications, these genetic variants are likely to affect LGALS ‐3 expression levels. In order to reach a definitive conclusion on the role played by rs2274273 and rs17128183 in advanced CA , our results should be further validated.