Open AccessRelationship between Xsp I Site Polymorphisms of LDL ‐R Gene and Serum IL ‐2 and IL ‐10 in Patients with Hypercholesterolemia
Author(s) -
Zhang Mingming,
Lu Yamin,
Liu Xin,
Zhang Xiaobin,
Zhang Cuigai,
Gao Wei,
Tie Yanqing
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21991
Subject(s) - genotype , medicine , polymorphism (computer science) , endocrinology , blood lipids , gene polymorphism , lipoprotein , familial hypercholesterolemia , interleukin , gene , cholesterol , biology , cytokine , genetics
Background Relationship has been identified in sporadic reports between polymorphisms and hypercholesterolemia. However, the relationship between inflammatory cytokines and polymorphism of low‐density lipoprotein receptor ( LDL ‐R) gene in hypercholesterolemia is unclear. This study aimed to explore the relationship and significance between polymorphisms of LDL ‐R gene and serum Interleukin‐2 ( IL ‐2), IL ‐10 in patients with hypercholesterolemia. Methods PCR ‐ RFLP and direct DNA sequencing assay were employed to determine polymorphism of LDL ‐R gene in 900 patients with hypercholesterolemia and 400 healthy cases. ELISA was applied to assay serum concentration of IL ‐2 and IL ‐10. Blood lipid indexes were tested in all cases. Results Compared with the healthy controls, level of IL ‐2 increased significantly, while IL ‐10 decreased significantly ( P < 0.05). Correlation analysis showed that IL ‐2 was positively correlated with total cholesterol ( TC ), LDL ‐c, and genotype ( r = 0.542, 0.410, 0.598, P < 0.05) and negatively correlated with HDL ‐c ( r = −0.352, P < 0.05). Negative relationship also was found between TC , LDL ‐c, genotype, and IL ‐10 ( r = −0.452, −0.390, −0.613, P < 0.05), and positive correlation between HDL ‐c and IL ‐10 ( r = 0.398, P < 0.05). Multiple linear regression showed that genotypes and TC were independent factors affecting the levels of IL ‐2 and IL ‐10 ( P < 0.05). Conclusion IL ‐2 and IL ‐10 were related to gene polymorphisms of LDL ‐R, which might be involved in the development and progress of hypercholesterolemia.