Open AccessHigh‐performance Liquid Chromatographic Ultraviolet Detection of Nilotinib in Human Plasma from Patients with Chronic Myelogenous Leukemia, and Comparison with Liquid Chromatography‒Tandem Mass Spectrometry
Author(s) -
Nakahara Ryosuke,
Satho Yuhki,
Itoh Hiroki
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21975
Subject(s) - nilotinib , chromatography , chemistry , detection limit , calibration curve , high performance liquid chromatography , liquid chromatography–mass spectrometry , mass spectrometry , tandem mass spectrometry , analytical chemistry (journal) , tyrosine kinase , biochemistry , signal transduction
Background A method for determining nilotinib concentration in human plasma is proposed using high‐performance liquid chromatography and ultraviolet detection. Materials & Methods Nilotinib and the internal standard dasatinib were separated using a mobile phase of 0.5% Na 2 PO 4 H 2 O (pH 2.5)‐acetonitrile‐methanol (55:25:20, v/v/v) on a Capcell Pak C18 MG II column (250 × 4.6 mm) at a flow rate of 1.0 ml/min, and ultraviolet measurement at 250 nm. Results The calibration curve exhibited linearity over the nilotinib concentration range of 50–2,500 ng/ml at 250 nm, with relative standard deviations ( n = 5) of 7.1%, 2.5%, and 2.9% for 250, 1,500, and 2,500 ng/ml, respectively. The detection limit for nilotinib was 5 ng/ml due to three blank determinations (ρ = 3). Conclusion This method was successfully applied to assaying nilotinib in human plasma samples from patients with chronic myelogenous leukemia. In addition, we compared the results with those measured by liquid chromatography‒tandem mass spectrometry ( LC ‐ MS / MS ) at BML, Inc. (a commercial laboratory). A strong correlation was observed between the nilotinib concentrations measured by our high‐performance liquid chromatographic method and those obtained by LC / MS ‐ MS ( r 2 = 0.988, P < 0.01).