Open AccessComparison Between an Automated and Manual Extraction for the Determination of Immunosuppressive Drugs Whole Blood Concentrations by Liquid Chromatography Tandem Mass Spectrometry
Author(s) -
Deslandes Guillaume,
Grégoire Matthieu,
Renaud Christian,
MonteilGanière Catherine,
Azoulay Claudine,
Pineau Alain,
Jolliet Pascale,
Dailly Eric
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21958
Subject(s) - automated method , chromatography , tandem mass spectrometry , liquid chromatography–mass spectrometry , extraction (chemistry) , mass spectrometry , chemistry , computer science , artificial intelligence
Background The whole blood extraction for liquid chromatography tandem mass spectrometry (LC‐MS/MS) of simultaneous quantification of cyclosporine A (Cys A), tacrolimus (Tacrs), sirolimus (Siros), and everolimus (Evers) is still performed manually in many laboratories. The analytical results obtained with an automated method using a liquid handler versus a classical manual preparation were compared. Methods Cys A ( n = 36), Tacrs ( n = 50), Siros ( n = 34), Evers ( n = 38) whole blood samples of patients were analyzed by LC‐MS/MS assay after manual preparation and automated process using a liquid handling platform including a centrifugation step. Results The comparison between manual and automated extraction investigated by a linear regression showed a high correlation between results [(Tacrs “automated”) = 1.0927 × (Tacrs “manual”) − 0.36; (Cys A “automated”) = 1.0284 × (Cys A “manual”) + 0.0312; (Siros “automated”) = 0.9923 × (Siros “manual”) + 0.4001; (Evers “automated”) = 1.0000 × (Evers “manual”) − 0.0600]. Conclusion The results obtained by the automated and manual preparation are consistent. The automated method is applied for high‐throughput therapeutic drug monitoring of immunosuppressive drugs in routine practice, leading to an increase in quality.