Open AccessUrinary Albumin Detection: Comparison of Two Different Methods
Author(s) -
Molinario Rossana,
Pocino Krizia,
Daloiso Pio Dante,
Giannace Angela,
Spirito Giulia,
Zuppi Cecilia,
Antenucci Mirca
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21952
Subject(s) - medicine , albumin , urinary system , roche diagnostics , clinical practice , urology , chromatography , chemistry , family medicine
Background Monitoring urinary albumin is a useful method in clinical practice for the management of diabetic nephropathy, chronic kidney disease, and hypertension. Currently there are neither standardized methods nor reference material for the determination of urinary albumin; for this reason it is useful to compare different assays used in clinical laboratory. Objectives The aim of this study is to verify analytical performance of an immunoturbidimetric assay on Roche Cobas 8000 platform and to compare urinary albumin results with those obtained by immunonephelometry on Siemens Dade Behring BN II Nephelometer. Results The method comparison showed a good linear relationship, confirmed by Passing–Bablok and Bland–Altman plots. The turbidimetric assay meets the requirements of accuracy and precision for the practice of medical diagnostics and clinical use. Conclusions The present study can contribute to the methods standardization and harmonization of urinary albumin assay.