Open AccessResazurin Microtiter Assay for Clarithromycin Susceptibility Testing of Clinical Isolates of Mycobacterium abscessus Group
Author(s) -
Carvalho Natalia Fernandes,
Sato Daisy Nakamura,
Pavan Fernando R.,
Ferrazoli Lucilaine,
Chimara Erica
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21933
Subject(s) - resazurin , mycobacterium abscessus , microbiology and biotechnology , clarithromycin , mycobacterium , minimum inhibitory concentration , microtiter plate , biology , chemistry , antibiotics , chromatography , bacteria , genetics
Background Mycobacterium abscessus group has heterogeneous susceptibility pattern among species. The species is most common cause of nosocomial infections. Macrolides minimum Inhibitory concentration (MIC) determination is essential for the treatment. Methods Thirty‐six strains were randomly selected for performing Resazurin Microtiter Assay (REMA) for clarithromycin testing in comparison to MIC test according to Clinical and Laboratory Standards Institute (2011) recommendation. REMA has been used for detection of drug resistance in M. tuberculosis . Extended incubation was performed to detect induced resistance. Results Thirty microliters of resazurin (0.01%) was added after visually taking MIC reading. Resistance was observed in 11.1% of M. bolletti and 4.8% of M. abscessus strains; and induced resistance was detected in 77.8% and 95.2% of M. bolletti and M. abscessus strains , respectively. All strains of M. massiliense were susceptible. The samples presented same MIC value both by visual reading and through resazurin. Conclusion The present study showed 100% concordance between both readings, with REMA providing easier to read and report results benefit. This change in reading can also reflect on the MIC determination and report, improving the test.