Open AccessEfficacy of an Enrichment Media for Increasing Threshold for Carbapenem‐Resistant Enterobacteriaceae Screening
Author(s) -
Peretz Avi,
Pastukh Nina,
Isakovich Natlya,
Koifman Anna,
Brodsky Diana,
Mizrahi Hila,
Aharon Ilana,
Labay Kozitta
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21903
Subject(s) - macconkey agar , microbiology and biotechnology , brain heart infusion , agar , imipenem , enterobacteriaceae , agar plate , biology , bacteria , antibiotics , escherichia coli , antibiotic resistance , biochemistry , genetics , gene
Background Identification of carbapenem‐resistant Enterobacteriaceae (CRE) is complex and a major laboratory challenge; clinical cultures may diagnose only some of the CRE carriers among patients, thus it is crucial to perform asymptomatic carriage screening. Materials and methods We compare the efficacy of a rectal sample culture prior to enrichment with BHI (Brain Heart Infusion) Broth and following 18–24 h. All rectal samples were applied on CHROMagar KPC selective growth media and then seeded on MacConkey agar selective growth media with an applied disk of Imipenem antibiotic on top of the media, then inserted into enrichment BHI Broth. After 18–24 h incubation with enrichment media, all samples were applied again on this media. Results From the 2,245 rectal samples, CRE colonies were found in 96 (4.3%). Following enrichment with BHI Broth, CRE colonies were found in 111 (4.9%) CHROMagar KPC plates and 106 (4.7%) MacConkey agar. Conclusion We were able to demonstrate that the number of CRE‐positive results increased due to use of additional enrichment with BHI Broth. Therefore, we recommend applying this method of addition of liquid enrichment media as part of a culture protocol routine for CRE screening.