Open AccessAccurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman‐ARMS Followed a Strict Control System
Author(s) -
Lu Renfei,
Shao Jianguo,
Zhou Min,
Zhang Hongping,
Wu Yueping
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21857
Subject(s) - taqman , hepatitis b virus , virology , virus , ligase chain reaction , real time polymerase chain reaction , population , biology , polymerase chain reaction , medicine , genetics , gene , multiplex polymerase chain reaction , environmental health
Background Hepatitis B virus (HBV) G1896A mutation was associated with HBeAg seronegativity and hepatitis B related acute‐on‐chronic liver failure. In this study, we developed Taqman amplification refractory mutation system (Taqman‐ARMS) and established a strict control system to detect HBV G1896A mutant. Methods HBV viral DNA was isolated from 60 patient serum samples, and full‐length HBV genome was cloned. Then, Taqman‐ARMS was used to detect HBV G1896A mutant. Results The assay has the sensitivity of 1E+3 IU/ml G1896A template, and 0.1% weak population virus with G1896A could be found in mixtures. Total of all 60 clinical samples random collected were detected by Taqman‐ARMS, the results were consistent with those by DNA sequencing. Conclusion The proposed Taqman‐ARMS real‐time PCR method for the detection of G1896A mutation of HBV was rapid, simple, sensitive, specific, and applicable in the clinical setting.