Open AccessTF‐Test Modified: New Diagnostic Tool for Human Enteroparasitosis
Author(s) -
Carvalho Juliana Barboza de,
Santos Bianca Martins dos,
Gomes Jancarlo Ferreira,
Suzuki Celso Tetsuo Nagase,
Hoshino Shimizu Sumie,
Falcão Alexandre Xavier,
Pierucci Julia Cestari,
Matos Lucas Vinicius Shigaki de,
Bresciani Katia Denise Saraiva
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21854
Subject(s) - test (biology) , medicine , population , diagnostic test , veterinary medicine , biology , environmental health , ecology
Intestinal parasitosis is highly prevalent worldwide, being among the main causes of illness and death in humans. Currently, laboratory diagnosis of the intestinal parasites is accomplished through manual technical procedures, mostly developed decades ago, which justifies the development of more sensitive and practical techniques. Therefore, the main objective of this study was to develop, evaluate, and validate a new parasitological technique referred to as TF‐Test Modified, in comparison to three conventional parasitological techniques: TF‐Test Conventional; Rugai, Mattos & Brisola; and Helm Test/Kato‐Katz. For this realization, we collected stool samples from 457 volunteers located in endemic areas of Campinas, São Paulo, Brazil, and statistically compared the techniques. Intestinal protozoa and helminths were detected qualitatively in 42.23% (193/457) of the volunteers by TF‐Test Modified technique, against 36.76% (168/457) by TF‐Test Conventional, 5.03% (23/457) by Helm Test/Kato‐Katz, and 4.16% (19/457) by Rugai, Mattos & Brisola. Furthermore, the new technique presented “almost perfect kappa” agreement in all evaluated parameters with 95% ( P < 0.05) of estimation. The current study showed that the TF‐Test Modified technique can be comprehensively used in the diagnosis of intestinal protozoa and helminths, and its greater diagnostic sensitivity should help improving the quality of laboratory diagnosis, population surveys, and control of intestinal parasites.