Open AccessHLA‐A, HLA‐B, and HLA‐DRB1 Allele and Haplotype Frequencies in Renal Transplant Candidates in a Population in Southern Brazil
Author(s) -
Saito Patrícia Keiko,
Yamakawa Roger Haruki,
Noguti Erika Noda,
Bedendo Gustavo Borelli,
Júnior Waldir Veríssimo da Silva,
Yamada Sérgio Seiji,
Borelli Sueli Donizete
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21846
Subject(s) - human leukocyte antigen , allele , haplotype , hla a , population , immunology , locus (genetics) , allele frequency , genetics , hla b antigens , biology , typing , histocompatibility testing , genotype , medicine , antigen , gene , environmental health
Background Very few studies have examined the diversity of human leukocyte antigens (HLA) in the Brazilian renal transplant candidates. Methods The frequencies of the HLA‐A, HLA‐B, and HLA‐DRB1 alleles, haplotypes and phenotypes were studied in 522 patients with chronic renal failure, renal transplant candidates, registered at the Transplant Centers in north/northwestern Paraná State, southern Brazil. Patients were classified according to the ethnic group (319 whites [Caucasians], 134 mestizos [mixed race descendants of Europeans, Africans, and Amerindians; browns or “pardos”] and 69 blacks). The HLA typing was performed by the polymerase chain reaction sequence‐specific oligonucleotide method (PCR‐SSO), combined with Luminex technology. Results In the analysis of the total samples, 20 HLA‐A, 32 HLA‐B, and 13 HLA‐DRB1 allele groups were identified. The most frequent allele groups for each HLA locus were HLA‐A*02 (25.4%), HLA‐B*44 (10.9%), and HLA‐DRB1*13 (13.9%). The most frequent haplotypes were HLA‐A*01‐B*08‐DRB1*03 (2.3%), A*02‐B*44‐DRB1*07 (1.2%), and A*03‐B*07‐DRB1*11 (1.0%). Significant differences ( P < 0.05) were observed in the HLA‐A*68 , B*08 , and B*58 allele frequencies among ethnic groups. Conclusions This study provides the first data on the HLA‐A, HLA‐B, and HLA‐DRB1 allele, phenotype and haplotype frequencies of renal transplant candidates in a population in southern Brazil.